Washington University Fish Facility Per diem and Services Fee Schedule


Pricing Effective July 2017

PER DIEM FEES

Adult Room  
    • 1.1 L tank $0.06 per diem
    • 3.5 L tank $0.18 per diem
    • 8 L tank $0.35 per diem
Nursery Room and Quarantine Room  
    • 1.1 L tank $0.12 per diem
    • 3.5 L tank $0.36 per diem
    • 8 L tank $0.70 per diem
Each month, an investigator's laboratory will be charged 1.5 times the per diem up to the first 60, 3.5 liter tank equivalents (one full rack) in adult rooms. The surcharge is to cover facility infrastructure, equipment and supply costs that do not simply scale up with the number of tanks. After the 60 tank threshold has been met, remaining tanks will be charged at the regular per diem, which covers fish maintenance.

OTHER SERVICES & FEES

Tank Exchange

REQUEST FORM

    • Fee is based on 80 3.5 L tanks per rack $70.00 per rack
Tank Maintenance

REQUEST FORM

    • 3.5 L tank standard $0.86 per month
Shipping & Receiving Embryos/Adult Fish  
    • Ship embryos, up to 4 lines Contact Dr. Isa Roszko for a shipping/receiving price quote
    • Ship adults, up to 2 lines
• Price does NOT include embryo generation or FedEx shipping cost
    • Receive embryos, up to 4 lines
    • Receive adults, up to 2 lines & includes anti-parasite treatment Contact John Engelhard for shipping/receiving address and FedEx help
If the receiver fails to use their own FedEx number and the Facility is charged, the user will be charged 1.5x the shipping cost to cover both shipment and administration fees.
Rotifers

REQUEST FORM

    • Contact Dr. Isa Roszko for discussion of shipping rotifers Contact Dr. Isa Roszko for a quote
Embryo Handling  
    • Up to 150 embryos $13.00 per 150 embyros
Crossing Fish

REQUEST FORM

    • User sets up cross or Facility staff sets up cross Contact Dr. Isa Roszko for a quote
    • Natural cross or in vitro fertilization
Bleaching and Dechorionation

REQUEST FORM

    • One line; handling of embryos is included $20.00 per 100 embryos
Cryopreservation of Line

REQUEST FORM

    • One line: 5 fish/10 vials $59.00 per line (10 vials)
Recovery of Line from Cryopreserved Sperm

REQUEST FORM

    • One line, includes set up of females $58.00 per line
Tissue Collection

REQUEST FORM

    • Embryo, finclip, or sperm Contact Dr. Isa Roszko for a quote
    • Fee is based on protocol used and sample size
DNA Isolation

REQUEST FORM

    • Lysis (embryo/finclip) or hotshot (sperm) Contact Dr. Isa Roszko for a quote
    • Fee is based on protocol used and sample size
Genotyping

REQUEST FORM

    • PCR/digest/gel/purification/extraction/sanger sequencing/deep sequencing/
      high resolution melt analysis/fragment length analysis
Contact Dr. Isa Roszko for a quote
    • Controls will also be included in sample size/quote calculation
    • PNACL and GEiC fees are included in the quote
Cloning

REQUEST FORM

    • Contact Dr. Jimann Shin for discussion of project Contact Dr. Jimann Shin for a quote
RNA Synthesis

REQUEST FORM

    • Contact Dr. Isa Roszko for discussion of project Contact Dr. Isa Roszko for a quote
Training

REQUEST FORM

    • Microinjection of embryos, includes supplies $82.00 per hour
    • Other training $41.00 per hour
Microinjection

REQUEST FORM

    • Up to four sample tubes $82.00 per hour
Experiments & Design/Consultation by FF Staff  
    • Contact Dr. Isa Roszko for discussion of project $41.00 per hour

Fee Schedule prices are subject to change without advance notice.